Difo Famiglia:
The Difo Famiglia is a Mafia Famiglia that runs in Asia. They were first seen at Mafia Land. They have good relationships with the Vongola Famiglia and seem to respect them due to the difference in size.

Todd Famiglia:
The Todd Famiglia is an ordinary Mafia Famiglia that attempted to capture Fuuta in hopes of obtaining hisRanking Book and powers; they were, however, easily defeated by Tsuna whilst he was in Dying Will Mode.

Estraneo Famiglia:
The Estraneo Famiglia was a normal Mafia Famiglia.Mukuro,Ken, andChikusawere born into this Famiglia. However, when they created the Possession Bullet, the entire Famiglia was labelled as brutal and its members were horribly persecuted by the other Mafia Famiglias. According to Ken, as soon as the members of the Famiglia stepped outside, guns were pointed at them, and they were killed like bugs. The killings just stimulated the adults of the Famiglia even more in continuing the experiments to invent special weapons and used the children of the family as "guinea pigs." One of the researchers of the Estraneo Famiglia stated that the invention of the special weapon was a stepping stone for the Famiglia to restore their fallen glory. He also said, "Think of it as a honor to die by being a part of the development." The children of the Famiglia were dying one by one as a result of the experiments.

Later, when Ken and Chikusa seemed to be the only surviving children left, in the middle of an experiment, Mukuro killed all of his captors with his Six Paths of Reincarnation. With Mukuro's rebellion, all the members of the Famiglia were killed, ending the Famiglia's existence.

Pesca Famiglia:
The Pesca Famiglia sent one of their assassins to attack Tsuna. The assassin used Cloud Flames to enlarge his own body. Tsuna went into Hyper Dying Will Mode, and while the assassin was distracted by Natsu, Tsuna defeated the assassin. According to Reborn, the assassin was in Hyper Dying Will Mode using an alternate version of the Dying Will Bullet.

Bertesca Famiglia:

The Bertesca Famiglia was allied to the Vongola Famiglia during both Famiglias' first generation. Their base and territory was located in Catafalco Island in Mediterranean, Italy. The Bertesca Primo got along well with the Vongola Primo, Giotto, and their Famiglias worked together to keep peace among their respective towns. However, this changed when someone named Parvento wrecked havoc, causing both Famiglias multiple problems. Unable to completely defeat Parvento, both the Bertesca Primo and Giotto sealed away Parvento by freezing him entirely with Zero Point Breakthrough: First Edition. To keep Parvento frozen forever, they created the Vongola Snow Ring, which was the key of the seven crystals scattered throughout the island that kept Parvento frozen permanently. Each crystals were placed inside a temple that guarded by seven main members of Bertesca Famiglia (excluding Bertesca boss who is guarding the frozen Parvento). The only way to free Parvento was to freeze the original Vongola Rings with the Vongola Snow Ring and have the frozen rings unfreeze by destroying the seven crystals that connected to the island's ice spikes. To do so would require the same type of Flame that sealed Parvento, the Sky Flame. In order to prevent Parvento's seal from being destroyed, the Bertesca Primo decided that he and his Famiglia, along with the villagers of the island, would stay on Catafalco Island to protect the seal and to prevent Parvento's tyranny from arising once more, thus cutting off their connection with the main civilization and the Vongola. Ever since then, the Bertesca Famiglia and the villagers of Catafalco Island diligently protected the seal from one generation to the next.

This decision, however, made the Bertesca Famiglia an pariah of the Mafia world and were thus persecuted by other mafia Famiglias due to their isolation on the island and their subsequent weakening, but never forget their main duty to protect the villagers and prevent Parvento from being revived again. Several years before the main storyline of the game, however, things started to change when Gelaro's father were killed during one of the persecutions, leaving Gelaro distraught and orphaned. In the middle of his grief, he came across the frozen Parvento during his walk to the underground part of his mansion. Recognizing Gelaro as Bertesca Primo's desecndant, he manipulated him to think that the Vongola was the cause of their weakness and that Vongola Primo only used them as a stepping stone, and that the only way to wipe out Vongola was to free him from his frozen state. As Parvento had hoped, Gelaro then decided to seek revenge on Vongola and convinced his remaining Famiglia members to fight against the Vongola and to free Parvento, not knowing what had actually occurred between the Bertesca and the Vongola and Parvento's evil intentions.

After Parvento was defeated and the misunderstandings were all cleared, Bertesca reconciled with Vongola and becoming their ally again.

Giegue Famiglia:

The Giegue Famiglia (nicknamed the "Grave Diggers") is an allied Mafia Famiglia to the Vongola Famiglia that hails from Russia. They planned to attend Vongola Decimo'sInheritance Ceremony in Japan. They have a fearsome reputation as a group of assassins, as Gokudera was nervous about his own appearance when he heard the Giegue Famiglia was coming to the Inheritance Ceremony.

They first appear when they discovered the location of the group attempting to interfere with the Ceremony. As a result, the Giegue Famiglia decided to deal with the interfering group as a gift to Vongola Decimo; however, they were defeated by an unknown female fighter, who, according to them, did not possess any of the conventional Dying Will Flames. Though the actual outcome of their battle with the mysterious fighter has yet to be confirmed, Reborn stated that their bodies were left in strange positions, implying that there were no survivors of the conflict. During the Inheritance Ceremony, the attacker was revealed to be Adelheid Suzukiof the Simon Famiglia, who used a Glacier Flame, a Dying Will Flame belonging to the Dying Will Flames of the Earthrather than the Dying Will Flames of the Sky.

Bovino Famiglia:

The Bovino Famiglia is only seen through the action of sending packages to Tsunayoshi Sawada and Shoichi Irie. Lambo is the only Bovino mafioso to make an appearance. The Bovino Famiglia is known as a weak Famiglia but, as stated by Reborn, has a powerful firearm arsenal.